Translational_Unit
AtIPT4

Part:BBa_K1139100:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-08-29)


Adenylate isopentenyltransferase 4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cds is exactly the same as IPT4 gene sequence of Arabidopsis thaliana.
The RBS is BBa_B0030 (efficiency 0.6).

Source

http://www.ncbi.nlm.nih.gov/gene/828567

References

1. Kentaro Takei, Hitoshi Sakakibara1, Mitsutaka Taniguchi and Tatsuo Sugiyama. (2001) Nitrogen-Dependent Accumulation of Cytokinins in Root and the Translocation to Leaf: Implication of Cytokinin Species that Induces Gene Expression of Maize Response Regulator. Plant Cell Physiol, 42, 85–93
2. Kentaro Takei, Hitoshi Sakakibara, and Tatsuo Sugiyama. (2001) Identification of Genes Encoding Adenylate Isopentenyltransferase, a Cytokinin Biosynthesis Enzyme, in Arabidopsis thaliana. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 276, 26405-26410